Substrates for the determination of enzyme activity and intermediates for the synthesis of these substrates as well as process of the production of the intermediates

摘要

Novel compounds represented by the following formula: ##STR1## wherein A represents a specific amino acid residue are excellent as substrates for determination of enzyme activity such as trypsin, etc. The compounds can be synthesized from novel arginyl-3-tert-alkyloxycarbonyl-4- nitroanilides by a novel process comprising a selective deprotection step whereby the protecting group on the &agr;-amine group of arginine is removed in the presence of a hydrochloric acid, acetic acid and dimethylformamide mixture, but a tert-alkyl protecting group on the -- COOH group of the nitroanilide ring is not removed by this step.