Sequences coding for the 29, and beta2 - beta3 - canine receptors and their applications as probes and for the expression of peptides

摘要

Nucleotide sequences coding for certain canine beta -adrenergic receptors, particularly the beta 2- (SEQ ID NO 1) and beta 3- (SEQ ID NO 24) receptors, as well as the use of said sequences as probes, for expressing canine beta -adrenergic receptors, for studying the tissue location of the expressed receptor (PCR, Northern blot), for carrying out directed mutagenesis, and for receptor structure-function studies, are disclosed. The use of said canine beta -adrenergic receptors for preparing specific antibodies is also disclosed. Furthermore, a differential screening method for affinity studies of substances having an agonist or antagonist activity for canine beta 3-adrenergic receptors, and kits for studying the effectiveness of various substances for said canine beta 3-adrenergic receptors, are disclosed. Such substances are particularly useful for selectively treating obesity or other obesity-related metabolic disorders in dogs, as well as any other diseases in which canine beta 3 receptors are involved.