Fermentation process for producing natamycin with additional carbon and nitrogen

摘要

An improved method for producing natamycin by fermentation is disclosed. In the method, which comprises, in order, the steps of: PP(a) introducing an inoculum of a natamycin producing Streptomyces species to a fermentation medium to produce a fermentation broth comprising the fermentation medium and inoculum; andP P(b) producing natamycin by fermentation in the fermentation broth; PPthe improvement comprising:PP(1) providing at least 15 g/L of a protein nitrogen source to the fermentation medium; wherein the protein nitrogen source comprises a non- yeast protein component and a yeast protein component, and the ratio of non-yeast nitrogen component to yeast protein component being from about 3:1 to 9:1, based on the protein content of the source;PP (2) providing a carbon source to the fermentation medium at from about 80 g/L to 250 g/L; wherein the carbon source addition is carried out during step (b) so as to maintain a concentration of carbon source of from about 5 g/L to 30 g/L; andPP(3) continuing the fermentation until the fermentation broth comprises at least 5 g/L natamycin.