Screening and characterization of glutaryl-7-aminocephalosporanic acid acylase

摘要

A method of screening for a given enzyme in colony-forming cells using a substrate of the enzyme that has low solubility. Use of such a method leads to the discovery of glutaryl-7-aminocephalosporanic acid (GL-7-ACA) acylase of Pseudomonas nitroreducens. Also disclosed is a method of obtaining GL-7-ACA acylase from Pseudomonas cells.