48 kD cellular phosphoprotein CtBP

摘要

Cloning of the cDNA for CtBP via yeast two hybrid interaction cloning. The DNA encodes a 439 amino acid (48 kD) protein that specifically interacts with exon 2. This protein requires residues 225- 238 of the 243R E1A protein for interaction. The predicted protein sequence of the isolated cDNA is identical to amino acid sequences obtained from peptides prepared from biochemically purified CtBP. Fine mapping of the CtBP- binding domain revealed that a six amino acid motif highly conserved among the E1A proteins of various human and animal adenoviruses is required for this interaction.