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Method of preferential labelling of a phycobiliprotein with an aminereactive dye for use in a multiple color assay and product for such use
Method of preferential labelling of a phycobiliprotein with an aminereactive dye for use in a multiple color assay and product for such use
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机译:用胺反应性染料优先标记藻胆蛋白的方法,用于多色分析及其产品
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摘要
A method for preparing phycobiliprotein/amine-reactive dye conjugates is disclosed in which the conjugates so prepared overcome the energy transfer/fluorescent quenching dilemma encountered in the use of prior art conjugates. A phycobiliprotein, for example, phycoerythrin or allophycocyanin, is conjugated with an amine-reactive dye, for example, Texas Red or carboxyfluorescein succinimidyl ester, in the presence of a selective salt which causes a hydrophobic intramolecular rearrangement of the phycobiliprotein thereby exposing more hydrophobic sites for binding to the amine-reactive dye. The conjugates prepared according to the invention are useful in multiple color fluorescence assays without requiring the use of multiple exciting sources.
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