NON-HUMAN ANIMAL EMBRYO STEM CELL HAVING VARIANT SMOOTH MUSCLE MYOSIN HEAVY CHAIN GENE AND NON-HUMAN ANIMAL

摘要

PROBLEM TO BE SOLVED: To obtain embryonic stem cells of a non-human mammalian or the cells deriv therefrom that is useful for the evaluation of drugs for arteriosclerosis, as a morbid state model and the like by including a mutant type smooth muscle myosin heavy chain gene that does not function as the chromosome- originating smooth muscle myosin heavy chain gene. SOLUTION: A part of chromosome-derived smooth muscle myosin heavy chain gene is deleted and an extraneous gene is inserted into the chromosome- derived smooth muscle myosin heavy chain gene, or the chromosome-derived smooth muscle myosin heavy chain gene is partially substituted with an extraneous gene, or these operations are combined to obtain the mutant type smooth muscle myosin heavy chain gene that has lost the function as the chromosome- derived smooth muscle myosin heavy chain gene. This mutant type gene is obtained by inserting a reporter gene as a β-galactosidase gene or the like into the chromosome-derived smooth muscle myosin heavy chain gene and the T-antigen gene is preferably transduced into the cells that are separated from a non-human mammalian and cultured.