MUTANT TYPE SECRETION DEVICE GENE THAT EFFICIENTLY SECRETS PROTEIN IN THE CELL BODIES OF CORYNEBACTERIUM

摘要

PROBLEM TO BE SOLVED: To provide the subject novel gene that comprises the DNA encoding a protein having a specific amino acid sequence in which the amino acid residue at a specific position is phenylalanine and can gives a secretory device that can efficiently secret a prescribed protein in the cell bodies of a Coryne form bacterium. ;SOLUTION: This DNA encodes the protein having an amino acid sequence corresponding to formula I in which the amino acid residue at the 44 position is replaced with phenylalanine. The secretor that is constituted as including the above protein is a novel DNA that can more efficiently secret the prescribed protein in Coryne form bacteria than the secretor that is constituted as including the protein having the amino acid sequence of formula II. This DNA is obtained by isolating the chromosomal DNA of a Brevibacteerium flavum MJ-233 (FERM BP-1497) through the hybridization technique using the oligo DNA probe and subjecting the isolated DNA to the site-specific mutation so that the amino acid at the 44-position becomes the residue of phenylalanine.;COPYRIGHT: (C)1999,JPO