Production of a heterologous polypeptide in the collapse function in the absence of mRNA by nonsense mediated

摘要

(57) and gene named NMD2 in honor of the role in the (N onsense- M ediated mRNA D ecay) route collapse of the mRNA by nonsense-mediated [summary] The present invention, discovery of protein Nmd2p encoded by NMD2 gene about. The amino acid sequence of Nmd2p, the nucleotide sequence of NMD2 gene encoding it are disclosed. As used herein, capable of binding to a protein Upf1p another in the collapse path Nmd2p are shown. Also, the C-terminal fragment of the protein, binds to Upf1p, when overexpressed in the host cell, to inhibit the function of Upf1p, whereby the fragment inhibits the decay path of the mRNA by nonsense-mediated. The present invention, in order to stabilize the mRNA transcript containing nonsense codon results in an increase in the disintegration rate of the transcript, to a method of inhibiting the decay path of the mRNA by nonsense-mediated normally. Such stabilization of the transcript are useful to produce fragments thereof, or recombinant proteins.