PROBLEM TO BE SOLVED: To obtain the subject medium capable of excellently proliferating a hair papilla cell in a serum-free state or low serum state, by adding an extract of pituitary body to a basal medium for animal cell culture. ;SOLUTION: An extract of pituitary body such as an essence of bovine pituitary body is added to a basal medium for animal cell culture (preferably αMEM medium or DMEM medium). Preferably insulin and/or transferrin besides the essence of bovine pituitary body is added to the medium. Preferably the amounts of the extract of pituitary body, insulin and transferrin added are 50 μg/mL, 5 μg/mL and about 5 μg/mL, respectively.;COPYRIGHT: (C)1999,JPO
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机译:解决的问题:通过将垂体的提取物添加到用于动物细胞培养的基础培养基中,获得能够在无血清状态或低血清状态下优异地增殖毛乳头细胞的主题培养基。 ;解决方案:将垂体提取物(例如牛垂体精华)添加到用于动物细胞培养的基础培养基(最好是αMEM培养基或DMEM培养基)中。优选地,除了牛垂体的本质之外,将胰岛素和/或转铁蛋白加入培养基中。优选地,垂体提取物,胰岛素和转铁蛋白的提取物的添加量分别为50μg/ mL,5μg/ mL和约5μg/ mL 。;版权所有:(C)1999,JPO
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