A lipase-containing detergent compositions and lipase and a method of manufacturing a microorganism to produce it and lipase

摘要

PURPOSE: To obtain a lipase having high stability to detergent components such as surfactant and protease, usable by compounding to a detergent together with a protease, resistant to the lowering of activity by the detergent components and capable of increasing the detergent power of the detergent. ;CONSTITUTION: This invention relates to a bacterial, strain belonging to the genus Pseudomonas, an alkaline lipase produced by the bacterial strain and having the following properties and a detergent composition containing the lipase. (1) The working pH is 3.5-12 and the optimum pH is 10-11 when a triolein emulsion is used as the substrate; (2) the working temperature is 30-80°C and the optimum temperature is 55-65°C when a triolein emulsion is used as the substrate; (3) the molecular weight is 31,000±2,000 determined by SDS- polyacrylamide get electrophoresis and (4) the isoelectric point is 5.2±0.5 determined by isoelectric polyacrylamide get electrophoresis.;COPYRIGHT: (C)1995,JPO