Obtaining of Pepsin from proventriculos (glandular stomachs) of chickens

摘要

A procedure for obtaining proventru00edculos Pepsin from Chicken, which comprises: a) a step of extraction,Where the proventru00edculos Chicken Frozen ground in a Grinder, subsequently mixed with an extractant Solution (pH = 7.4) containing Sodium PhosphateSODIUM benzoate, Rice Husk Powder is added after a period of Rest after mixture obtained by Filtering a Liquid; (b) a saturation stage.Where the liquid obtained by Filtration is saturated up to a 25% p / p with Solid AMMONIUM SULFATE by Continuous agitation, leave to rest between 16 and 18 hours at a temperature of between 4 and 10 c,The resulting solution was centrifuged at 5000 rpm for collecting the supernatant precipitation Stage; (c) a isoelu00e9ctrica and ActivationWhere the liquid obtained is adjusted to a pH between 3 and 4, adding hydrochloric acid to increase its temperature 30 and 95 which produces an abundant precipitation,Letting it Rest for 30 minutes until the temperature drops to between 20 and 25; (d) a step of proteu00f3losis and purificationWhere the solution precipitated from the previous Phase (Liquid Solid) is adjusted to a pH of between 1.8 and 2.0, was left to stand at room temperature for no more than an hour,The solution obtained was centrifuged at 5000 rpm, the supernatant was discarded and the pellet was resuspended in water elevu00e1ndole pH between 6.5 - 7.0 by addition of Sodium hydroxide and stirring at room temperature until complete dissolution of sediment...