Nucleic acid sequence encoding an amino acid variant of human plasminogen, expression vector and host cell comprising the same, as well as a method for producing an amino acid variant of plasminogen and a method for producing a binary complex between a fibrinolytic ...

摘要

A cleavage-resistant plasminogen molecule is provided that is conveniently produced in recombinant cells by expression of a nucleic acid sequence encoding the plasminogen molecule. Preferably the plasminogen is a sequence variant with a modification in its two-chain cleavage site. The plasminogen molecule may be purified, acylated, complexed with acylated or non-acylated fibrinolytic enzymes, and formulated into pharmaceutical compositions for use in thrombolytic therapy.