NUCLEIC ACID SEQUENCE HAVING DELTA-5,7 STEROL, DELTA-7 REDUCTASE ACTIVITY, A METHOD FOR PRODUCING PROTEIN, A METHOD FOR RECOVERY OF STEROLS UNSATURATED AT THE POSITION C-7, A METHOD FOR PRODUCING PREGNENOLONE, TRANSFORMATED STRAIN SACCAROMYCES CEREVISIAE ACCUMMULATING FREE AND ETHERIFICATED PREGNENOLONE AND A METHOD FOR REVEALING THE LACK OF DELTA-5,7 STEROL, DELTA-7 REDUCTASE

摘要

DNA sequence coding protein which has delta-5,7 sterol, delta-7 reductase activity, protein which has delta-5,7 sterol, delta-7 reductase activity, isolated of rabidopsis thaliana, and also a method for producing the said DNA sequence and protein. Disclosed are different methods for recovery of sterols unsaturated at the position C-7, methods of producing pregnenolone, transformed yeast strain Saccaromyces cerevisiae, accumulating free and etherificated pregnenolone, and also a method for revealing the lack of delta-5,7 sterol, delta-7 reductase.