Nitrate reductase applicable for the detection of nitrates at high temperature thermoset.

摘要

NITRATE REDUCTASE THERMOSTABLE APPLY FOR DETECTING NITRATE HIGH TEMPERATURES, which involves cloning the DNA region encoding nitrate reductase THERMOSTABLE APPLY FOR DETECTING NITRATE HIGH TEMPERATURE FROM A DNA library of the bacterium Thermus thermophilus HB8 , using specific DNA probe for detection. AN EXCERPT FROM 8.2 kbp, between two PSTI restriction sites was isolated from in this region and cloned into the vector bifunctional PMK18. TABLE PMK18 is characterized by having two origins of replication FUNCTIONAL Escherichia coli and Thermus thermophilus, a gene conferring resistance THERMOSTABLE kanamycin PROTETIDO BY Invention Patent 9,301,522 REQUESTED IN SPAIN, a promoter inducible in ESCHERICHIA COLI AND A REGION OF CLONING WITH MULTIPLES DOS restriction enzyme sites. WHEN construct is introduced into Thermus thermophilus HB27 OR IN ESCHERICHIA COLI, SESINTETIZA SOLUBLE an enzyme SIZE OF MOLECULAR 192 kilodaltons, capable of catalyzing the reduction of nitrate or nitrite to an optimum temperature of 80 C.