Expression of human serum albumin in Pichia pastoris.

摘要

NEW CASSETTE, EXPRESSION VECTORS AND PROCESSES FOR SECTION human serum albumin in Pichia pastoris cells. According to the present invention it has discovered an expression cassette IMPROVED FOR PRODUCING human serum albumin in Pichia pastoris comprising (A) a regulatory region 5 '' THE Pichia pastoris having one end 5 '' and an end 3 '' SELECTED GROUP consisting of oleosin 5 'AOX1 OF Pichia pastoris and region REGULADORA 5' 'DAS1 OF Pichia pastoris WHERE YOU END 3' 'region REGULADORA 5' 'is linked operably A: (B) UN STRUCTURAL GENE human serum albumin encoding a signal sequence and a protein MATURE having one end 5 '' and an end 3 '' wherein the structural gene OF human serum albumin has an initial ATG Within 11 deoxyribonucleotides aPPROXIMATELY END 5 '' said structural gene for human serum albumin; And is linked operably AC) ENDING A SEQUENCE 3 ''.