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Device and methods for purifying biological molecules using low volumes of chromatographic and adsorptive media
Device and methods for purifying biological molecules using low volumes of chromatographic and adsorptive media
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机译:使用少量色谱和吸附介质纯化生物分子的装置和方法
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摘要
A device and methods for purification of biological molecules are disclosed. The device comprises a volume, 30 mu L or less, of an adsorptive media (1) disposed over a volume, 100 mu L or less, of a chromatographic media (2). Preferably, the adsorptive media is an ion exchange media and the chromatographic media is a desalting media. The media may be packed in a single housing or in separate housings (4, 4), which due to the small volume of media, can be an individual well in a multiwell device. Methods for purification of biological molecules using the device are also disclosed. In accordance with the principles of the invention, a biological sample is introduced into the device, wherein molecules of interest are adsorbed onto the adsorptive media, then a volume of elution buffer, preferably less than or equal to one-third the volume of the second chromatographic media, is used to elute the desired molecules from the adsorptive media, wherein the resulting elutant passes into the second chromatographic media for collection.
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