Colorimetric assessment of the sensitivity of (helicobacter) (pylori) to antimicrobial substances

摘要

A colourimetric method which is advantageously applicable to a culture medium of the liquid type, and which is further suitable for the evaluation in vitro of sensitivity and resistance of Helicobacter pylori to antimicrobial pharmaceuticals, is based on the colourimetric detection of bacterial growth of Helicobacter pylori stemming from an increase in bacterial urease concentration, said colour variation being made possible by a pH colour indicator injected into the culture medium. A colourimetric kit for the assessment of Helicobacter pylori's sensitivity and/or resistance to antimicrobial pharmaceuticals, said assessment being carried out with the naked eye and/or by spectrophotometric means, comprises: a) a plurality of microwells and/or vessels made of transparent material and containing predetermined antimicrobial substances and at suitably predetermined concentrations, said vessels being advantageously assembled in printed modules and further being packed in a sterile manner; b) a culture medium for Helicobacter pylori containing urea and a colour pH indicator.