首页>
外国专利>
A method for isolating a polynucleotide of interest from the genome of a mycobacterium using a bac-based dna library. application to the detection of mycobacteria
A method for isolating a polynucleotide of interest from the genome of a mycobacterium using a bac-based dna library. application to the detection of mycobacteria
The present invention is directed to a method for isolating a polynucleotide of interest that is present or is expressed in a genome of a first mycobacterium strain and that is absent or altered in a genome of a second mycobacterium strain which is different from the first mycobacterium strain using a bacterial artificail chromosome (BAC) vector. The invention further relates to a polynucleotide isolated by this method and recombinant BAC vector used in this method. In addition the present invention comprises method and kit for detecting the presence of a mycobacteria in a biological sample.
展开▼