The hemoglobin content of a sample is determined by chemiluminescence, based on the ability of hemoglobin to absorb radiation emitted by a chemiluminescent reaction. To perform the assay, the sample is placed in a liquid medium with a compound that is susceptible to a chemiluminescent reaction, and the medium is exposed to conditions causing the chemiluminescent reaction to occur. The amount of emission created by the reaction and not absorbed by the hemoglobin is then detected and compared to calibrators or standards as a measure of the amount of hemoglobin present. By combining the above with procedures that separate and quantify glycosylated hemoglobin, the assay can be used to determine glycosylated hemoglobin as a proportion of the total.
展开▼
