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ANTIBODIES OR BINDING PROTEIN LIBRARIES DISPLAYED ON PHAGE, CELLS, OR OTHER REPLICATABLE GENETIC PACKAGES
ANTIBODIES OR BINDING PROTEIN LIBRARIES DISPLAYED ON PHAGE, CELLS, OR OTHER REPLICATABLE GENETIC PACKAGES
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机译:噬菌体,细胞或其他可替代基因包装上显示的抗体或结合蛋白文库
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摘要
The invention is directed to inter allia two related but self-sufficient improvements in conventional display methods. The first improvement provides methods of enriching conventional display libraries for members displaying more than one copy of a polypeptide prior to affinity screening of such libraries with a target of interest. These methods can achieve diverse populations in which the vast majority of members retaining full-length coding sequences encode polypeptides having specific affinity for the target. In a second aspect, the invention provides methods of subcloning nucleic acids encoding displayed polypeptides of enriched libraries from a display vector to an expression vector without the need for clonal isolation of individual members. These methods result in polyclonal libraries of antibodies and other polypeptides for use, e.g., as diagnostics or therapeutic reagents.
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