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GENE TARGETING AND SIBLING-SELECTION TO GENERATE MUTANT INDIVIDUALS
GENE TARGETING AND SIBLING-SELECTION TO GENERATE MUTANT INDIVIDUALS
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机译:基因突变和单株选择以产生突变个体
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摘要
The invention relates to a method to generate defined mutant individuals from parent organisms with a known gene. In particular the invention relates to a method to generate defined mutant individuals from multi-cellular parent organisms where homologous recombination is not easily applicable e.g. because embryonal stem cells or oocytes cannot easily be handled. In addition, the invention relates to a method wherein the parent organism is a uni-cellular organism such as a bacterium or other micro-organism or a virus, especially when these micro-organisms cannot easily be transfected or transduced and are thus less accessible to homologous recombination techniques. The mutant individual originates from a parent organism that is mutagenized and the mutant individual is selected by sibling-selection via amplification of or hybridisation with at least a part of the known but now mutagenized gene of offspring of the mutagenized parent organism.
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