REAGENTS FOR TESTING CLEANNESS AND METHODS FOR TESTING CLEANNESS

摘要

Reagents for testing cleanness, which permit the measurement of not only ATP serving as an indication of stain but also ADP, AMP and RNA difficultly measurable by the luciferin-luciferase luminescence reagents or the prior art, and which enable the detection even of a slight stain at high sensitivity and the evaluation of cleanness at high accuracy; and methods of testing cleanness with the same. The methods comprise bringing a stain-collecting support into contact with the place to be tested to recover the support bearing a stain adhering thereto, bringing aseptic water or an extractant for microbial intracellular components into contact with the resulting support, reacting the solution thus obtained with (1) a reagent for testing cleanness comprising pyruvate orthophosphate dikinase, phosphoenolpyruvic acid, pyrophosphoric acid, luciferin, luciferase and a metal salt or (2) a reagent for testing cleanness obtained by adding to the reagent (1) at least one member selected from the group consisting of enzymes catalyzing the formation of ATP from ADP and ribonucleases, and measuring the quantity of luminescence.