METHODS AND COMPOSITIONS FOR IMPROVING THE SUCCESS OF CELL TRANSPLANTATION IN A HOST

摘要

The present invention covers significant improvements for each event involved in the transplantation success or graft survival. These improvements, separately or combined with each other, greatly ameliorate the recovery of a tissue towards a normal function. They comprise: a) the reduction of early death of transplanted cells by anti-inflammatory agents such as TGFbeta1, an inhibitor of oligosaccharide synthesis, a glucosidase, IL-10, vIL-10, IL-4, INFgamma, IL-2R, IL-1Ra, Fas-L, sCR1, a super oxide dismutase, a neutrophil inhibitory factor (NIF), a ligand binding in an antagonist fashion to LFA-1, MAC-1, ICAM-1, CD-18, CD-31, CD-50, E-selectin, P-selectin, TNFalpha, IL-1 and IL-8. The anti-inflammatory agents may comprise an anti-LFA-1 or -ICAM-1; b) the improvement of the diffusion and of the fusion of transplanted cells with the host tissue by metalloproteases; c) the ex vivo proliferation of the transplanted cells with growth factors or oncogenes; d) the use of fibroblasts or stem cells in lieu of myoblasts, by transforming the formers into the latter with myogenic genes; e) expressing utrophin in lieu of dystrophin in cases of muscular dystrophy; and e) immunosuppressing the host for long-term graft survival.