A process for the production of alpha-human atrial natriuretic polypeptide

摘要

A process for the production of alpha -human atrial natriuretic polypeptide ( alpha -hANP) involves firstly culturing a microorganism which has been transformed with an expression vector comprising a synthetic gene encoding the amino acid sequence of a protective peptide-fused alpha -hANP in a culture medium. Subsequently recovering the protective peptide-fused alpha -hANP from the culture broth and then removing the protective peptide part from the peptide-fused polypeptide. In addition to the process itself, chemically synthesised genes for alpha -hANP and protective peptide-fused alpha -hANP are also described together with the corresponding recombinant vectors and transformants comprising the same. alpha -hANP is known to be useful as an antihypertensive diuretic agent.