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USE OF LEUKAEMIA INHIBITORY FACTOR (LIF) FOR THE (IN VITRO) PROPAGATION OF EMBRYONIC STEM CELLS
USE OF LEUKAEMIA INHIBITORY FACTOR (LIF) FOR THE (IN VITRO) PROPAGATION OF EMBRYONIC STEM CELLS
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机译:白血病抑制因子(LIF)在胚胎干细胞体外繁殖中的应用
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PCT No. PCT/AU89/00330 Sec. 371 Date May 31, 1990 Sec. 102(e) Date May 31, 1990 PCT Filed Aug. 3, 1989 PCT Pub. No. WO90/01541 PCT Pub. Date Feb. 22, 1990.The present invention relates generally to the use of leukaemia inhibitory factor (LIF) in the maintenance and derivation of embryonic stem (ES) cells in culture. The ES cells are maintained and/or derived from animal embryos by culturing said cells or embryos in a culture medium containing an effective amount of LIF for a time and under conditions sufficient to maintain and/or derive said ES cells. The ES cells may be passaged in LIF and used to make chimaeric animals.
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