CIRCULAR, HAIRPIN, CIRCULAR/HAIRPIN, LARIAT, AND HAIRPIN-LARIAT HAMMERHEAD RIBOZYMES

摘要

Hammerhead ribozyme derivatives termed circular, hairpin, circular/hairpin, lariat, and hairpin-lariat ribozymes are disclosed. These hammerhead ribozyme derivatives are catalytically active and have increased specific activity and reduced requirement for divalent metal ion co-factor as compared to wild type hammerhead ribozyme. These derivatives can be used as antisense therapeutic compositions.