Expression of the alpha-toxin gene (hla) of staphylococcus aureus using a chromosomally encoded hla-lacZ gene fusion

摘要

The present invention relates to a Staphylococcus aureus strain containing an reporter gene operatively linked to the promoter of the hla-gene, wherein the reporter gene is the lacZ gene; a bacterial strain, obtainable by transforming staphylococcus aureus wood 46 with hla-lacZ fusion gene using the recombinant plasmid pkO10 carrying said fusion gene, the bacterial strain Staphyloccocus aureus Wood 46-3; the use of a bacterial strain according to any of the proceeding claims to measure the hla promoter activity under different growth conditions, wherein the different growth conditions are selected from the group consisting of length of growth time and phase, temperature, osmolarity, glucose concentration, solid/liquid media, oxygen concentration, and concentration of low, subinhibitory concentrations of antibiotics; the vector pkO10, and a process for the production of Staphylococcus aureus 46-3.