RECOMBINANT STRAIN OF GENE GENERATING AMYLASE AND METHOD FOR PRODUCING AMYLASE BY USING STRAIN

摘要

The present invention relates to an amylase-producing gene recombinant strain and a method for producing maltooligosaccharide-producing amylase using the same.;The present invention is to clone the amylase gene from the strain Streptomyces KSM-35 strain producing maltotetraose-producing amylase to make a plasmid and to prepare a plasmid deleted gene expression related to expression and secretion using restriction enzymes , A recombinant plasmid with high expression and secretion capacity in Bacillus subtilis, which contains amylase gene, and at the same time, creates a new plasmid with high amylase secretion ability and injects into Bacillus subtilis LKS88 to significantly improve amylase production capacity. Transformant Bacillus subtilis KFCC-10880 strain and a method for producing amylase by fermentation using the transformant, which is a useful method to obtain amylase in high yield.