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PROCEDURE FOR STORING RED CELLS WITH PROLONGED MAINTENANCE OF CELLULAR CONCENTRATIONS OF ATP AND 2,3 DPG
PROCEDURE FOR STORING RED CELLS WITH PROLONGED MAINTENANCE OF CELLULAR CONCENTRATIONS OF ATP AND 2,3 DPG
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机译:ATP和2,3 DPG细胞浓度长期维持的红细胞储存方法
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摘要
This invention provides an improved method for prolonging the shelf life of transfusible red blood cells by decreasing the effective osmolality of the suspending solution and increasing the intracellular pH of the cells prior to storage thereof. This invention also provides methods whereby the intracellular pH may be increased. These methods include collecting the cells in an anticoagulant at pH 7.0 or higher and/or washing, diluting or resuspending the cells prior to storage thereof in a biologically compatible buffered solution that contains at least one non-penetrating or substantially non-penetrating anion or non-electrolyte and is substantially free of chloride ions.
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