How to Identify the Inhibitory Ability of Inhibitors against Myogenic Activated Protein Kinase Kanease

摘要

The present invention relates to a method for confirming the activity of protein kinase kinase by reacting protein kinase kinase with its substrate protein, radioactive isotope labeled ATP and an inhibitor for the enzyme and adsorbing it to a filter. Specifically, the present invention relates to a method for producing a microorganism, which comprises reacting a mitogen activated protein kinase kinase (MEK-1), which is important for cell signal transduction, with an active protein kinase, ATP, which is a substrate protein, in an activated form and reacting the reaction product with a nitrocellulose filter Followed by sensitization thereof to confirm the ability of the inhibitor to inhibit the MEK-1 protein.