Improved method for producing polyunsaturated proteasome sub-unit vaccines that are non-covalently conjugated

摘要

To a method for preparing a polyproteosome-amphibolite crystallizer vaccine suitable for parenteral administration or mucosal administration using dialysis or ultrafiltration techniques. This amphotriol determinant includes gram negative bacteria such as, for example, Flexosin, Flocomonas siegeloids and lipopolysaccharide from fungus. Proteomas are obtained from group B type 2b meningococci. The active proteosome-amphoteric crystallite complex (noncovalent complex salt) of this vaccine is formed using dialysis or ultrafiltration to remove the detergent. The use of this dialysis or ultrafiltration can shorten the process time and reduce the probability of contamination, and further enable the use of atmospheric temperature and useful bulking. This process also allows reliable and continuous observation of the dialysate to improve the efficiency of the entire process. Dialysis times for large vaccine preparations are shortened from 7-10 days to less than 72 hours and usually less than 48 or 24 hours. The use of this process is to optimize the presence of each antigen component in the production of a multivalent vaccine.