Rotavirus antigens, vaccines against rotavirus infections, diagnostic agents and methods for producing antigens

摘要

The present invention relates to a method for producing a cell clone-purified rotavirus strain by cloning cells highly tolerated by a large-scale cultivation of rotavirus from cultured cells, purifying the obtained clonal cell line with the rotavirus, A method for producing a rotavirus antigen, which comprises culturing the strain as a seed virus, which is prepared by using a rotavirus strain or a strain obtained by using the strain as a seed virus, and isolating and purifying the rotavirus antigen from the culture, A vaccine for rotavirus infection prepared using the antigen, and a diagnostic agent. These antigen vaccines and diagnostic agents can contribute greatly to the basic research and clinical applications of rotavirus infection.