Method for measuring protease activity isolated from Turnip Mosaic Virus C5

摘要

The present invention relates to a method for measuring the activity of a protease isolated from Mosaic virus C5 (Turnip Mosaic Virus C5, hereinafter abbreviated as TuMV-C5), which is a kind of potyvirus.;Specifically, the present invention is a method of measuring the activity of protease at the C-terminus of NIa protein, which plays an important role in viral proliferation by being involved in the maturation process of polyprotein expressed in TuMV-C5, wherein the amino acid to which the protease can act The present invention relates to a method for determining the activity of a protease simply, quickly and accurately by identifying a sequence, synthesizing the peptide, and using the peptide as a substrate.