METHOD FOR MEASURING ACTIVITY OF PROPHENOL OXIDASE ACTIVATING ENZYME APPLICATION

摘要

General formula:;Wherein X is an optionally labeled amino acid residue or peptide residue having an optionally protected α-amino group or N-terminus, provided that the amino acid residues adjacent to Arg are not Gly or Ala, and Y is an acid amide bond or An organic residue capable of binding to the carboxyl end of Arg by an ester bond, or an optionally labeled amino acid residue or peptide residue having an optionally protected α-carboxyl or C-terminus) and also derived from insects. Measuring at least one of X (a) rg or Y produced by contacting PPAE to a peptide chain that can be hydrolyzed to X (a) rg and Y by a phenol oxidase activating enzyme (PPAE) Method for measuring PPAE activity. According to the present invention, PPAE activity can be accurately quantitatively analyzed, and a very accurate method for measuring β-1, 3-glucan and / or peptidoglycan can be provided in which the PPAE activity is used as an indicator. In addition, the present invention is capable of detecting fungi and bacteria without using a culture method, thereby enabling early diagnosis of microbial infections. The present invention can be used in a wide range of applications such as microbial contamination testing of water and foods, safety testing of therapeutic agents such as antibiotics and injections.