Recombinant Rhodobacter sphaeroides 3-hydroxy fatty acid dehydrogenase gene and methods of production

摘要

An isolated DNA molecule (I) comprising a structural gene that encodes a protein with the enzymatic activity of a 3-hydroxy fatty acid dehydrogenase (3-HBDH) from a microorganism, is new. Independent claims are also included for: (1) a recombinant DNA, that is capable of replicating in a microorganism, comprising a plasmid vector and (I); (2) a microorganism transformed with a recombinant DNA as above; (3) a method for the production of a protein with 3-HBDH activity; (4) an isolated protein with a 3-HBDH activity; and (5) a method for the enzymatic characterization of ketone bodies present in a biological sample.