Resistance of p53 mutant cancer cells to cytoxic effects of (chemo)therapeutic agents involving assay of cyclin D1 protein

摘要

The measurement of the resistance of p53 cancer cells to the cytotoxic effects of chemotherapeutic agents comprises assay of a sample comprising p53 mutant cells, or an extract therefrom, for the level of cyclin D1 protein. The agent may be a platination agent, especially CDDT (cis-diaminedichloro-platinum). The level of cyclin D1 may be determined either from the mRNA thereof, especially involving a probe therefor, or by contacting the sample with a labelled antibody against cyclin D1 protein. Mutant cells may be identified using an antibody against mutant p53. A kit, for performing the process, is described.