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Design and synthesis of bispecific reagents: use of double stranded DNAs as chemically and spatially defined cross-linkers
Design and synthesis of bispecific reagents: use of double stranded DNAs as chemically and spatially defined cross-linkers
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机译:双特异性试剂的设计和合成:使用双链DNA作为化学和空间定义的交联剂
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摘要
The invention relates to bis-protein-DNA conjugates. A protein having a specific ligand binding activity is covalently linked to each end of a derivatized DNA molecule. These bis-protein-DNA conjugates can be used for immunoassays, PCR assays and measuring distances between proteins at up to 3.4 A resolution. The invention also relates to methods of synthesizing these bis-protein-DNA conjugates. Synthesis of the conjugates entails derivatizing the 5' or 3' end of a DNA oligonucleotide and covalently linking that DNA to a protein. The DNA can be conjugated to the proteins, including antibodies or Fab' fragments, using disulfide bond linkage.
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