Process for manufacturing a xylanase enzyme complex from pre- treated thin stillage of rye

摘要

A process is disclosed for manufacturing a xylanase-rich enzyme complex by cultivating a xylanase-producing microorganism of the genus Trichoderma in a nutrient medium involves cultivating said microorganism in a nutrient medium containing a pre-treated thin stillage of rye as both the xylanase inductor and the carbon source. Pre-treatment of the thin stillage comprises removing the solid constituents of the thin stillage of rye, concentrating the non-volatile components by evaporation of water and other volatile substances, and autoclaving the thin stillage of rye concentrate resulting therefrom. De-oiled soya meal or soya meal liquor can be used as an additional xylanase inductor and as a nitrogen source. Addition of the de-oiled soya meal or soya meal liquor to the pre- treated thin stillage of rye further increases the xylanase production. The enzyme complexes so manufactured can be used immediately in the animal feedstuff industry such as poultry feeds. The use of said enzyme complexes in rye-, barley- or triticale-containing feedstuffs reduces the antinutritive action of the non-starch polysaccharides, leading to improved digestability and absorption of the nutrients in an animal's intestine.