Method for measuring the affinity for chemicals of protein during the screening of combinatorial libraries

摘要

Measurement of affinity for chemicals protein target (57) during screening of [summary] combinatorial library. The synthesized on a solid support having a gradient of coupling site library, and to have a compound concentration within a certain range target. I is measured by quantitative imaging analysis the distribution of protein bound to the immobilized compound. The Rankuzukeru in order of binding affinity by comparison of the ability to bind across the gradient, and compound.