It depends on modifying nutrient medium temperature while culturing the plant cell, pakuritakiseru (paclitaxel) mass production

摘要

(57) Abstract This invention while culturing the plant cell, pakuritakiseru (paclitaxel) regards the method of increasing production level by modifying the temperature. With this invention, as for production of pakuritakiseru, in the (i) nutrient medium, the process which cultures the yew being attached plant cell, with approximately 20-25C; And at the point in time when multiplication of that plant cell (ii) advanced sufficiently, approximately 26-32 C modifying culture temperature, the process which continues culture; Including, it becomes. In addition, this invention pakuritakiseru production includes the method of increasing by inserting the early cell of high density, and making the saccharide density in the nutrient medium high. With this invention, it to be possible to produce pakuritakiseru, simply in large quantities, therefore, this method can be applied industrially.