Cloning and revelation of the gene which the secondary alcohol dehydrogenase of samoanearobakuta etanorikasu 39E the cord/code is done and the biochemical characterizing of the enzyme

摘要

(57) Abstract The secondary alcohol dehydrogenase of samoanearobakuta etanorikasu 39E (2-Adh) cloning it did the adhB gene which the cord/code is done, it arranged decided, made reveal next in the colon bacillus. The cord/code are done both of nuclear acid arrangement and the amino acid arrangement which are reported the enzyme. The rearrangement enzyme which was refined is the homo- four quantitative bodies which are formed from the sub unit of 37.7kDa, is optimum activity at the temperature which exceeds the 90C, it possesses the half-life of approximately 1.7 hours with the 90C. The enzyme primary alcohol compared to likes the oxidation of the secondary alcohol substantially.