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Equipment and fractionation method using a field-flow fractionation and dielectric electrophoresis

机译:使用场流分离和介电电泳的设备和分离方法

摘要

(57) [Abstract] The present invention relates to a novel method for separating materials, characterization, and new equipment for the operation. In particular, in order to identify and separate such materials, the present invention combines the conductive properties of the dielectric and frequency dependence of the dissolved material and the particulate material, the use of the characteristics of suspended transport medium. For introducing the cells, is taken out, the apparatus includes a chamber and an inlet and outlet ports and at least one electrode element of at least one. Then, material carried through the chamber by the flow of fluid is separated by a fluid (DEP) force dielectric electrophoresis caused by electrodes energized. Following separation in the fluid, material moves through the chamber at a speed corresponding to the speed profile. After the material has passed through the chamber, the material exits from the opposite end of the chamber at distinctive locations. The method of the present invention, comprises the use of research, diagnosis of a condition, the apparatus for separating and identifying the substance for therapeutic purposes. Examples of such methods include the separation of red blood cells isolated from normal cells of cancer cells, parasitized erythrocytes from normal, such as the separation of nucleic acids, the separation of the cell mixture.
机译:(57)[摘要]本发明涉及一种分离材料的新颖方法,特征和用于该操作的新设备。特别地,为了识别和分离这样的材料,本发明结合了电介质的导电特性以及溶解的材料和颗粒材料的频率依赖性,悬浮运输介质的特性的使用。为了引入细胞,将其取出,该设备包括腔室,入口和出口以及至少一个的至少一个电极元件。然后,由流体的流动携带通过腔室的材料被由电极通电引起的流体(DEP)力介电电泳分离。在流体中分离之后,材料以对应于速度曲线的速度通过腔室。物料通过腔室后,物料从腔室的另一端在不同的位置排出。本发明的方法包括使用研究,病症诊断,用于治疗目的的分离和鉴定物质的设备。这样的方法的例子包括从癌细胞的正常细胞分离的红细胞的分离,从正常细胞中被寄生的红细胞的分离,例如核酸的分离,细胞混合物的分离。

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