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DIFFERENTIATION INDUCTION OF PRIMARY PRECURSOR ADIPOSE CELL, AND CULTURE MEDIUM FOR ITS DIFFERENTIATION
DIFFERENTIATION INDUCTION OF PRIMARY PRECURSOR ADIPOSE CELL, AND CULTURE MEDIUM FOR ITS DIFFERENTIATION
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机译:原代前脂肪细胞的分化诱导及其分化培养基
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摘要
PROBLEM TO BE SOLVED: To evaluate therapeutic agents for obesity, diabetes and the like in no need of consideration on the influence of serum components by inducing the differentiation of the primary precursor adipose cells to adipose cells in a nutrient medium containing insulin or the like, selenious acid or its salt, indomethacin or the like.;SOLUTION: The differentiation of a primary precursor adipose cells to the adipose cells is induced in a nutrient culture medium selected from among the Eagle basal medium (MEM), α-Eagle basal medium (αMEM), Dulbecco- modified Eagle medium (DMEM), and Ham' F-12 medium, including insulin, transferrin, dexamethasone, biotn, ascorbic acid, glucose, epidermal growth factor, or fibroblast growth factor, and selenious acid or its salts, in addition at least one selected from the group consisting of indomethacin, prostaglandin, long-chain fatty acid and thiazolidine derivative. In a preferred embodiment, ≤3 wt.% of serum is added to the nutrient culture.;COPYRIGHT: (C)2000,JPO
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