Fixation method of microbe, fixation membrane, and BOD of microbe or measuring method and measurement equipment null

摘要

PURPOSE: To obtain a fixed microorganismic membrane having sufficient response, sufficient sensitivity, stability and durability, and useful as a biological device, etc., of a sulfuric acid detector by fixing a microorganism to a hydrophilic porous membrane having a specified average pore diameter using a gelling agent. ;CONSTITUTION: Microorganisms are fixed to a hydrophilic porous membrane (e.g. membrane made of acetylcellulose for membrane filtration) having 0.65 to 3μm average pore diameter by using a gelling agent (Alginic acid, agar-agar, etc., are preferably used). Specifically, washed microorganismic cells are mixed with a gelling agent solution and the resultant microorganism-containing gelling agent solution is dropped on the porous membrane. The porous membrane is then sucked from its back surface or pressurized from the upper side so as to infiltrate the above-mentioned solution into the holes of the porous membrane. Thereby, the microorganisms are fixed together with the gelling agent.;COPYRIGHT: (C)1993,JPO&Japio