MASS-PRODUCTION OF SEEDLING BODY BY CELL CULTURE OF ELEUTHEROCOCCUS SENTICOSUS MAX AND STORAGE OF SEEDLING BODY

摘要

PROBLEM TO BE SOLVED: To carry out the mass culture and mass-produce a seedling body of sterile and uncontaminated Eleutherococcus senticosus Max by carrying out the subculture of a specific embryogenic cell in a Murashige-Skoog(MS) liquid culture medium without containing a plant growth regulator added thereto, growing the cell into a uniform somatic cell embryo, transferring the resultant somatic cell embryo to an air feeding type bioreactor and then culturing the somatic cell embryo. ;SOLUTION: The subculture of an embryogenic cell KCTC 0504BP is carried out in an MS liquid culture medium without containing a plant growth regulator added thereto and grown to a uniform somatic cell embryo in the culture of a seedling body of the Eleutherococcus senticosus Max. The resultant somatic cell embryo is then transferred to an air feeding type bioreactor and cultured to afford the seedling body. At this time, the subculture of the somatic cell embryo of the Eleutherococcus senticosus Max can be conducted at an interval of 2-3 weeks to grow the somatic cell embryo into the somatic cell embryo of a uniform torpedo shape or in the cotyledon-shape period.;COPYRIGHT: (C)2000,JPO