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CHITINASE INHIBITOR AND PRODUCTION OF CHITINASE INHIBITING SUBSTANCE
CHITINASE INHIBITOR AND PRODUCTION OF CHITINASE INHIBITING SUBSTANCE
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机译:几丁质酶抑制剂和几丁质酶抑制物质的生产
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摘要
PROBLEM TO BE SOLVED: To prepare a chitinase inhibitor useful for antibacterial agents, insecticides, agrochemicals, ship bottom coatings for inhibiting the adhesion of living things, or the like by including a specific compound, as an active principle, obtained through culturing Isochrysis galbana belonging to Haptophyceae. ;SOLUTION: This compound of the formula is obtained by filling culture medium having a seawater composition and including nutrient into plastic bottles, by inoculating minute algae belonging to Isochrysis galbana belonging to Haptophyceae as spawn, by incubating the algae at 25°C for 2-3 weeks, by centrifuging the culture solution to collect alga body, by freeze-drying it, by adding acetone to freeze-dried product to subject it to extraction, by concentrating the extract to remove acetone, by adding ethyl acetate and water to the concentrate to perform the partition of the solute, by concentrating ethyl acetate layer to perform the partition with hexane and 70%- methanol, by purifying the 70%-methanol fraction through column chromatography. This chitinase inhibitor is produced by the preparation of the above compound and is useful for antibacterial agents, insecticides, a grochemicals, ship bottom coatings, or the like.;COPYRIGHT: (C)2000,JPO
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