Production method of cyclosporin A by high productivity cell fusion mutants

摘要

(57) Abstract This invention toripokuradeiumu inhurateyumu which produces the cyclosporin A which is the immunity inhibiter at high concentration (Tolypocladium inflatum) being something regarding the cell fusion variant, toripokuradeiumu inhurateyumu KD01 which is the parent strain which it picked from the soil makes the cell fusion body between each nutrition required stocks of the amino acid L valine and the L leucine which it mutates suddenly with the ultraviolet ray and the cell fusion stocks KD461 which feature that the L valine and the L leucine and required Characteristic and the utilization Characteristic which of nitrogen source of the organic state are the outrider material of the cyclosporin A increase, produce the abundant cyclosporin A were selected. As for fusion stocks KD461, on the solid media of the extract of the malt - yeast, the bacteriums reproduction some is slow in comparison with the parent strain, color of the colony thin with gray, to be denser back of the colony has the feature which has dark brown. In addition, with culture of the liquid the hypha some being thick briefly, formation of the segmentation spore is many. The L valine and the L leucine and the amount required of nitrogen source of the organic state it adds to amino acid increase, show the fact that it is the new variant where you show the feature where the generation tonnage of the cyclosporin A augments largely, you have the generation efficiency of the cyclosporin A of approximately 50 times in comparison with the parent strain, are different from the parent strain.