Development of end-pregnancy of animals from enuncleated oocytes reconstituted with adult somatic cell nuclei

摘要

Invention patent: b "development of pregnancy to term of animals from oocytes enucleados reconstituu00ecdos with adult somatic cell nuclei" d .The animals are produced after injection of adult somatic cell nuclei into oocytes enucleados.The invention provides a process for cloning an animal by direct insertion of at least a part of a nucleus of adult somatic cell oocyte in a enucleado container.Preferably, the core is inserted by micro injection molding and, more preferably, micro injection excited piezo electrically.The oocyte is activated before, during, or up to about 6 hours after insertion of the core, electro activation or exposure to a chemical agent ativante, as Mr ^ 2 + ^.The oocyte renucleado activated left to develop in the embryo and transplanted into a surrogate host to develop into a living child.