A method and Optimization on Protocols for DNA Amplification, Mixing and termociclacion Program, the identification of Genetic Polymorphisms of pit-1 Gene.The level of Milk Production and Fertility in cattle.

摘要

A method and Optimization of protocols for obtaining DNA from animal cells, as well as,The Optimization of the mix of programs and terciclacion amplifications of the pcr-rflp technique in the identification of Genetic polymorphism of pit-1 Gene in Milk Production and degreePreferably Fertility in Cows, characterized in that it comprises the extraction of DNA in milk samples and Hair follicleThe application of termociclacion the pit-1 gene amplification.Allowing the Amplification Fragment of 451 BP (BP) for the AA genotype and Fragments of 207 244 base Pair (BP) for the genotypeBB, were amplified, digested and resolved by electrophoresis in agarose gel.